Morphological and morphometric characteristics of fresh and cryopreserved spermatozoa of saanen bucks

Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout year to effectively increase livestock. Aim. To evaluate influence seasons on morphological and morphometric fresh cryopreserved sperm. Methods. The ejaculate samples three sexually mature bucks Saanen breed were obtained during non-breeding seasons. assess characteristics, smears sperm stained then visualized under a light microscope magnification ×1,000. For measurements, micrographs taken analyzed using ImageJ software. cryopreservation was performed medium 10 % glycerol 20 egg yolk. thawed water bath, cells evaluated. Results. After cryopreservation, number abnormal structures significantly higher compared sperm, both (p ≤ 0.05). length width head decreased 0.05) corresponding sizes before cryopreservation. Analyzing char- acteristics tail part, we revealed fact its reduction after probably due twisting, loop formation, detachment part. Conclusions. It found that season, damaged increases, which must be into account when technologies. buck change regardless indicates need choose fertilization tactics will ensure selection without any abnormalities.